Liofilchem Microbiology Products – Solutions for Antimicrobial Resistance Management

26 Aug 2022 Microbiology , ,

RPMI Agar

for antifungal susceptibility testing

RPMI Agar (RPMI1640, MOPS, L-glutamine, 2% Glucose) is intended for the AFST (antifungal susceptibility testing) and is recommended for usage with Antifungal MTS™ (MIC Test Strip).

Pick 4-5 colonies from 24-hours old cultures and suspend them in saline solution or sterile water in order to obtain a 0.5 McFarland cell density.
Introduce a sterile swab into the suspension and inoculate the RPMI Agar plate.

Allow the plate to dry, and position the antifungal strip onto the agar surface with the maximum concentration nearest the periphery of the plate.

Incubate at 35 °C for 24-48 hours.

Read the M.I.C. value where the edge of the inhibition ellipse intersects the strip (the intersection between two scale segments should be round up to the higher value).

Available ready-to-use formats:

Ref. 11509
90 mm agar plate
20/pack

Ref. 10233
140 mm agar plate
10/pack

10-25°C storage temperature
180 days shelf life

Inbox us today for more information and to place your orders!

Liofilchem Microbiology Products (Antimicrobial Resistance Management)

15 Jul 2022 Microbiology , , ,

Mueller Hinton Fastidious Broth and ComASP Benzylpenicillin

Mueller Hinton Fastidious Broth (MH-F broth) consists of ready-to-use, cation-adjusted MH broth supplemented with 5% lysed horse blood and 20 mg/L β-NAD. This medium is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) for MIC determination by the broth microdilution method of Streptococcus spp. (including S. pneumoniae), Haemophilus influenzae, Moraxella   catarrhalis, Listeria monocytogenes, Campylobacter jejuni and coli, Pasteurella multocida, Corynebacterium spp., Aerococcus sanguinicola and urinae, Kingella kingae and several other fastidious organisms.

new 11 mL tubes – 20/pack – ref. 21105
3.6 mL tubes (suitable for ComASP) – 20/pack – ref. 27507
2-8°C storage temperature
4 months shelf life

ComASP® Benzylpenicillin 0.002-32 µg/mL is an in-vitro diagnostic device for the quantitative determination of the minimum inhibitory concentration (MIC) of benzylpenicillin against Streptococcus pneumoniae.

ref. 75011
6 panels (2 tests/panel)
2-8°C storage temperature
16 months shelf life

ComASP® are Compact Antimicrobial Susceptibility Panel for determining MIC through broth microdilution according to ISO 20776-1:2019 standard, available both in a preset of antibiotic ranges and customizable assortments of agents and concentration ranges to meet the special needs of laboratories of any sizes.

 

WL Nutrient Agar

Medium for the cultivation and enumeration of yeasts, moulds and bacteria in the brewing and other fermentation industries.

WL (Wallerstein Laboratory) Nutrient Agar is a medium used for the cultivation and enumeration of fungal and bacterial species that can be encountered in beer brewing and other industrial fermentations. This medium meets the formula developed by Green and Gray while studying various fermentation processes.

Ref. 163712
20 plates/pack
10-25°C storage temperature
6 months shelf life

 

Quanti-CultiControl

for growth promotion testing of culture media

The Quanti-CultiControl are lyophilized, quantitative microorganism preparations to be used in industrial laboratories for Quality Control purposes. Processed as directed, these preparations provide a challenge of

<100 CFU per 0.1 mL, which is a concentration usually suitable for several pharmaceutical applications, including growth promotion testing of culture media to be employed in sterility testing. These microorganism preparations are derived from the American Type Culture Collection (ATCC®).

The Quanti-CultiControl are suitable for the Growth Promotion Test recommended by the international Pharmacopoeia, without the need for intermediate dilution steps, since each rehydrated suspension generates less than 100 UFC for 0.1 mL inoculum. Each suspension (1 mL) allows 10 inocula.

Liofilchem – Solutions for Antimicrobial Resistance Management

1 Jul 2022 Microbiology , ,

Ready-to-use Agar Dilution panels

With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin. Although several commercial tests based on different techniques have been developed, Agar Dilution (AD) is considered the best method for performing Fosfomycin susceptibility testing so far.

The AD Fosfomycin 0.25-256 is the first and only (international patent pending) ready-to-use commercially available kit for performing the agar dilution antimicrobial susceptibility testing, in compliance with the CLSI and EUCAST standards, with 4 months shelf life at present.

The AD Fosfomycin panel comprises 12 wells, containing the antibiotic incorporated into an agar medium in different concentrations, i.e. 11 two-fold dilutions (0.25-256 μg/mL).

Fosfomycin Agar Dilution panel
ref. 77001 – 1 panel/pack
ref. 77061 – 6 panels/pack

 

Chromatic CRE

for detecting Carbapenem-Resistant Enterobacteriaceae.

Chromatic CRE contains a mixture of carbapenems for screening a wide variety of carbapenem-resistance mechanisms and provides presumptive identification of E. coli and the Klebsiella, Enterobacter, Serratia and Citrobacter (KESC) group directly from clinical specimens.

Carbapenems, successfully used to treat multi-resistant Gram-negative bacterial infections, including ESBL positive strains, are not efficacious against the Enterobacteriaceae resistant to carbapenems, thus generating a significant risk of hospital-acquired infections.

Ready to use agar plates:

Ref. 11619
20/pack
90 days shelf life
2-8°C storage temperature

Dehydrated powder + supplement:
Ref. 611619
500 g
+ Chromatic CRE Supplement
Ref. 81088
10 vials/pack

Boditech Interleukin-6

3 Jun 2022 Immunology ,

Interleukin-6

The earliest alarming for sepsis

Every minute and every second, the medical team had always encountered challenges. The clinicians kept thinking about how to detect a baby’s condition earlier and how to reduce antibiotics administration. Interleukin-6 acts as a pro-inflammation cytokine and a key mediator of inflammation. During the acute phase of infection, B and T lymphocytes are stimulated to produce the Interleukin-6 cytokine, which in turn induces hepatocyte production of acute-phase reactants such as CRP.

With these characteristics, the Interleukin-6 could help to detect EOS(Early Onset Sepsis) earlier and can work as a complementary function with existing tests such as CRP(C-Reactive Protein) and PCT(Procalcitonin). Boditech Med has launched the AFIAS Interleukin-6 in September 2020, and it has been landed successfully. It is expected that the AFIAS Interleukin-6 test aid to detect inflammation in the early stage and will contribute to improving the newborn’s health.

 

Interleukin-6, an essential test for NICU and children’s hospital

1. Early Onset Sepsis occurs within 72 hours of life

Neonatal sepsis is a major contributor to morbidity and mortality in neonates, especially in those born preterm. The Global Burden of Disease (GBD) estimated 1.3 million annual incident cases of neonatal sepsis and other infections 2,874 sepsis cases per 100,000 live births and a mortality of 17.6%.

2. Exposure to inappropriate and/or excessive antibiotic use

Antibiotics are the most commonly prescribed class of medications in the NICU. The probability of EOS combined with the clinical instability characteristic of premature newborns leads to high rates of empirical antibiotic treatment. previous studies demonstrate that neonatal clinicians administer antibiotics from birth in 79% to 98%.

3. C-tip is the ideal method for infants’ or children’s sample collection

An infant’s blood collection cannot exceed 1% of body blood, usually premature baby is under 2 kg therefore, 1 ml for once is maximum. The research shows, that 51.7% of hospitals in Korea collect 0.5 ml, and 24.1% collect 1 ml from an infant’s heel for a blood culture test. It is already in the dangerous range for infants.

NICU clinicians need the method to improve diagnostic capabilities, which can provide reliable results, early and rapid, simple and safely in the patient’s bedside or in the ICU.

AFIAS parameters

ichroma parameters

Liofilchem Microbiology products (Antimicrobial resistance management)

2 Jun 2022 Microbiology , ,

MTS™ Synergy Application System
for in-vitro combination of antibiotics

Antimicrobial resistance is a major challenge for clinicians and clinical microbiologists, and the use of synergy testing is nowadays increasingly requested.

MTS-SAS combines the reliability of MIC Test Strip with patented tools to facilitate and standardize a critical assay such as the in-vitro combination of antibiotics.

MTS-SAS procedure is significantly faster than any other methods in antibiotic synergy tests (i.e. time-kill, checkerboard), does not require any additional training to those who are skilled in the usage of MIC Test Strip, reduces or eliminates the risks of procedure errors when compared to laborious and complicated traditional methods:

• standardized
• easy to use
• save time, materials, workload, resources

 

 

Individually packed, gamma-irradiated contact plates

The peelable blister pack fulfils the highest quality and protection standards for the plate.
The blister pack, fully automatically produced in a controlled environment, delivers the following benefits:

individual plate packaging;
clear package transparency;
drastically reduces the dehydration of culture media during the shelf life period;
up to 9 months shelf-life;
storage at ambient temperature for most of the items in its range;
reduces contamination risks in controlled environments;
available as gamma-irradiated, triple-enveloped plates, suitable for restricted areas such as isolators and clean rooms.

 

Liofilchem Microbiology products

20 May 2022 Microbiology , , , ,

(New) Fastidious Anaerobe Agar (FAA)

Fastidious Anaerobe Agar (catalogue ref. 10062) is a ready-to-use agar plate for the cultivation and

antimicrobial susceptibility testing of anaerobic bacteria. This medium is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) for disk diffusion of selected rapidly growing anaerobic bacteria, i.e. Bacteroides spp., Prevotella spp., Fusobacterium necrophorum, Clostridium perfringens and Cutibacterium acnes.

FAA permits excellent growth of most anaerobic bacteria and is the medium chosen by EUCAST for the development of a disk diffusion method for anaerobes.

FAA plates are inoculated with a bacterial suspension in 0.85% saline from overnight cultures, adhering to the 15-15-15-minute rule (suspension used within 15 minutes, disks applied within 15 minutes of inoculation, and incubation within 15 minutes of applying the disks). The antibiotic disks are applied to the agar surface. Plates are incubated in an inverted position at 36 ± 1°C for 16-20 hours in an anaerobic atmosphere (80% N2, 10% H2, 10% CO2).

At the end of the incubation period, the inhibition zone diameters are measured and interpreted according to the current EUCAST breakpoints and QC criteria for disk diffusion of anaerobes. Refer to the current EUCAST disk diffusion reading guide for anaerobic bacteria.

Ref. 10062
90 mm plates, 20/pack
2 months shelf life
2-8°C storage temperature

 

Enterosystem 18R

Biochemical identification of Gram-negative, oxidase-negative Enterobacteria

Enterosystem 18R is an 18-well system containing desiccated biochemical substrata for the identification of Gram-negative, oxidase negative enterobacteria. The system is inoculated with the suspension of the organism and incubated at 36±1°C for 12-18-24 hours. The microorganism is then identified by assessing the colour change in the wells to determine the corresponding numerical code. A codebook or the online Identification Code Disk allow for the microbial ID.

Ref. 71618
90 panels/pack
16 months shelf life
2-8°C storage temperature

 

Steel Coupons

for hydrogen peroxide sterilization

Biological indicators OXI CONTROL GST E6/E5/E4/E3/E2 SS are used for regular control of H2O

 

2 vapour superficial decontamination cycles and validation of sterilizers. These biological indicators use a stainless-steel support (coupon), that is not permeable to hydrogen peroxide. Stainless-steel coupon has been inoculated with

Geobacillus stearothermophilus ATCC® 7953 spores and has been introduced into a Tyvek®/Polytene envelope. Tyvek® side of the envelope is permeable to hydrogen peroxide vapour, while the Polytene side of the envelope is not permeable to these vapours. Biological indicators OXI CONTROL GST E6/E5/E4/E3/E2 are produced under strictly controlled conditions to satisfy the requirements indicated in the USP and EP current editions and accordance with ISO 11138 and EN 866 standards.

 

ref. 91082

20 coupons + 20 tubes
2-8°C storage temperature
3 years shelf life

 

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