Tag Archives: Liofilchem Microbiology products

(New) Fastidious Anaerobe Agar (FAA)

Fastidious Anaerobe Agar (catalogue ref. 10062) is a ready-to-use agar plate for the cultivation and

antimicrobial susceptibility testing of anaerobic bacteria. This medium is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) for disk diffusion of selected rapidly growing anaerobic bacteria, i.e. Bacteroides spp., Prevotella spp., Fusobacterium necrophorum, Clostridium perfringens and Cutibacterium acnes.

FAA permits excellent growth of most anaerobic bacteria and is the medium chosen by EUCAST for the development of a disk diffusion method for anaerobes.

FAA plates are inoculated with a bacterial suspension in 0.85% saline from overnight cultures, adhering to the 15-15-15-minute rule (suspension used within 15 minutes, disks applied within 15 minutes of inoculation, and incubation within 15 minutes of applying the disks). The antibiotic disks are applied to the agar surface. Plates are incubated in an inverted position at 36 ± 1°C for 16-20 hours in an anaerobic atmosphere (80% N2, 10% H2, 10% CO2).

At the end of the incubation period, the inhibition zone diameters are measured and interpreted according to the current EUCAST breakpoints and QC criteria for disk diffusion of anaerobes. Refer to the current EUCAST disk diffusion reading guide for anaerobic bacteria.

Ref. 10062
90 mm plates, 20/pack
2 months shelf life
2-8°C storage temperature

 

Enterosystem 18R

Biochemical identification of Gram-negative, oxidase-negative Enterobacteria

Enterosystem 18R is an 18-well system containing desiccated biochemical substrata for the identification of Gram-negative, oxidase negative enterobacteria. The system is inoculated with the suspension of the organism and incubated at 36±1°C for 12-18-24 hours. The microorganism is then identified by assessing the colour change in the wells to determine the corresponding numerical code. A codebook or the online Identification Code Disk allow for the microbial ID.

Ref. 71618
90 panels/pack
16 months shelf life
2-8°C storage temperature

 

Steel Coupons

for hydrogen peroxide sterilization

Biological indicators OXI CONTROL GST E6/E5/E4/E3/E2 SS are used for regular control of H2O

 

2 vapour superficial decontamination cycles and validation of sterilizers. These biological indicators use a stainless-steel support (coupon), that is not permeable to hydrogen peroxide. Stainless-steel coupon has been inoculated with

Geobacillus stearothermophilus ATCC® 7953 spores and has been introduced into a Tyvek®/Polytene envelope. Tyvek® side of the envelope is permeable to hydrogen peroxide vapour, while the Polytene side of the envelope is not permeable to these vapours. Biological indicators OXI CONTROL GST E6/E5/E4/E3/E2 are produced under strictly controlled conditions to satisfy the requirements indicated in the USP and EP current editions and accordance with ISO 11138 and EN 866 standards.

 

ref. 91082

20 coupons + 20 tubes
2-8°C storage temperature
3 years shelf life